History and development of microbiology
Development of microbiology with reference to modern era, important discoveries, theories of spontaneous
generation and germ theory of disease (Louis Pasteur, Robert Koch)

Classification and nomenclature of microorganisms
Classification schemes of living organism, differential characteristics of prokaryotic and eukaryotic
microorganism. General principle of nomenclature, three and five kingdom concepts, basic understanding of
classification of bacteria, viruses, fungi and protozoa, classification, nomenclature and characterization of
bacteria according to Bergey’s Manual of Systematic Bacteriology

Scope and applications of microbiology
Harmful and beneficial microorganism; applied microbiology (medical, public health, agricultural, food,
microbial biotechnology, environmental, industrial, pharmaceutical microbiology)
Morphology of bacteria 10hrs
Structure of bacteria, morphology and fine structure of cell organelles, differences between gram negative
and gram positive bacteria

Growth and physiology of bacteria

Nutritional types of bacteria (photolithotrophic, chemolithotrophic, photoorganotrophic,
chemoorganotrophic), entry of nutrients, passive and active transport, bacterial growth, growth curve,
factors affecting growth

Isolation, enumeration and culture of bacteria

Types of bacteriological culture medium, techniques for isolation and enumeration of bacteria (streak plate
technique, pour plate technique, spread plate technique, membrane filtration, most probable number
method, direct microscopic count), biochemical tests of bacteria, methods of culture of aerobic and anaerobic bacteria, culture preservation methods